Regulatory
PgpdA

Part:BBa_K678024:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)

PgpdA, promoter for Aspergillus nidulans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 140
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 140
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 140
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1116
    Illegal SapI site found at 322


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.

Source

Aspergillus nidulans genomic DNA

References